Phytochemical Content Analysis of 4 Marigold (Tagetes Erecta) Accsessions in Bengkulu Province Which has the Potential as a Poultry Feed Supplement

Authors

  • Wismalinda Rita Universitas Bengkulu
  • Mohammad Chozin Universitas Bengkulu
  • Sumardi Universitas Bengkulu
  • Dwatmadji Universitas Bengkulu

DOI:

https://doi.org/10.55927/ijis.v4i5.254

Keywords:

Marigold Flowers, Functional Compounds, Bioactive Compounds, GC-MS, Natural Feed Supplements

Abstract

Feed is the main factor that determines the success of livestock farming, both in terms of quality and quantity of livestock production. Functional feed containing bioactive compounds can improve health, endurance, and livestock productivity. Marigold flowers (Tagetes erecta L.) are known to contain bioactive compounds such as flavonoids, terpenoids, and carotenoids that have potential pharmacological activities such as antioxidants, antimicrobials, and immunostimulants. Four marigold accessions (Tagetes erecta) were used, namely 1. WRRL (Orange crown with small size); 2. WRLB1 (Yellow crown with wide size) ; 3. WRLB2 (Orange crown with small upper part and wide lower part) ; 4. WRRM ( Orange crown with wide size). Bioactive compounds were extracted using ethanol and analyzed by Gas Chromatography-Mass Spectrometry (GC-MS) method. The results of the analysis showed the presence of dominant compounds such as beta-Amyrin, Caprolactam, Vitamin E, and 9,12-Octadecadienoic acid which have the potential to have anti-inflammatory, antioxidant, and antibacterial activities. It is hoped that the results of this study can support the development of functional feed based on natural ingredients as an alternative to synthetic additives in the poultry farming industry that is more environmentally friendly and sustainable

References

Ao, X., & Kim, I. H. (2020). Effects of plant extracts as antibiotic alternatives on growth performance and intestinal health in broiler chickens. Animal Bioscience, 33(3), 447–454. https://doi.org/10.5713/ajas.19.0187

Aziz, M. A., Adnan, M., Khan, A. H., Shahat, A. A., Al-Said, M. S., & Ullah, R. (2017). Chemical composition and biological properties of Nigella sativa Linn. oil: A review. Arabian Journal of Chemistry, 10(1), e1180-e1188. https://doi.org/10.1016/j.arabjc.2013.12.002

Bakkali, F., Averbeck, S., Averbeck, D., & Idaomar, M. (2008). Biological effects of essential oils – A review. Food and Chemical Toxicology, 46(2), 446–475. https://doi.org/10.1016/j.fct.2007.09.106

Bouic, P. J. D. (2001). Sterols and sterolins: New drugs for the immune system? Drug Discovery Today, 6(14), 775–778. https://doi.org/10.1016/S1359-6446(01)01819-3

Brigelius-Flohé, R., & Traber, M. G. (1999). Vitamin E: Function and metabolism. The FASEB Journal, 13(10), 1145-1155. https://doi.org/10.1096/fasebj.13.10.1145

Chakraborty, M., & Mitra, A. (2008). The defensive role of plant triterpenoids in nature. Acta Botanica Hungarica, 50(1–2), 33–50. https://doi.org/10.1556/ABot.50.2008.1-2.5

Crozier, A., Clifford, M. N., & Ashihara, H. (2006). Plant Secondary Metabolites: Occurrence, Structure and Role in the Human Diet. Blackwell Publishing.

Dewick, P. M. (2002). Medicinal Natural Products: A Biosynthetic Approach (2nd ed.). Wiley.

El-Gengaihi, S. E., Hamed, A. I., & El-Shamy, A. R. (2013). Chemical composition and biological activity of hexacosane isolated from Nigella sativa seeds. Research Journal of Medicinal Plant, 7(3), 124–131.

El-Gengaihi, S. E., Nassar, M. I., El-Khrisy, E. A. M., et al. (2013). Antibacterial and insect-repellent activities of aliphatic hydrocarbons from plants. Journal of Applied Sciences Research, 9(1), 107–114.

Garba, I. H., & Samuel, B. B. (2011). Antimicrobial activity of n-Hexadecanoic acid isolated from the methanolic extract of Indigofera spicata. Journal of Medicinal Plants Research, 5(9), 1771–1775.

Garba, S. H., & Samuel, E. (2011). Antibacterial activity of n-Hexadecanoic acid extracted from the root bark of Terminalia avicennioides. Journal of Pharmacognosy and Phytotherapy, 3(6), 80–83.

Giri, A., Roy, R., Saha, A., & Halder, S. (2020). Phytochemical screening and pharmacological evaluation of marigold (Tagetes erecta L.): A review. Journal of Pharmacognosy and Phytochemistry, 9(2), 1469–1475.

Harborne, J. B. (1998). Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis (3rd ed.). Springer.

Hashemipour, H.; Kermanshahi, H.; Golian, A.; Veldkamp, T. Effect of thymol and carvacrol feed supplementation on performance, antioxidant enzyme activities, fatty acid composition, digestive enzyme activities, and immune response in broiler chickens. Poult. Sci. 2013, 92, 2059–2069. [Google Scholar] [CrossRef] [PubMed]

Jäger, S., Trojan, H., Kopp, T., Laszczyk, M. N., & Scheffler, A. (2009). Pentacyclic triterpene distribution in various plants—rich sources for a new group of multi-potent plant extracts. Molecules, 14(6), 2016-2031. https://doi.org/10.3390/molecules14062016

Kamal-Eldin, A., & Shahidi, F. (2002). Oxidative stability of muscle lipids of rainbow trout in the presence of organosulfur compounds and general antioxidants. Journal of Food Lipids, 9(1), 23–39. https://doi.org/10.1111/j.1745-4522.2002.tb00207.x

Kamboj, V. P., & Saluja, A. K. (2012). Pharmacological potentials of bioactive compounds from natural sources. Pharmacology Research & Perspectives, 1(2), 1-16.

Kelly, G. S. (1999). Squalene and its potential clinical uses. Alternative Medicine Review, 4(1), 29–36. https://www.altmedrev.com/archive/publications/4/1/29.pdf

Kumar, R., Chauhan, A., & Kumari, R. (2020). Synthesis and pharmacological potential of caprolactam and its analogs: A review. Current Drug Research Reviews, 12(3), 212–222.

Kumar, V., Singh, R., & Sharma, A. (2020). GC-MS Analysis of Bioactive Compounds from Medicinal Plants. Journal of Pharmacognosy, 34(2), 123-134.

Kumar, V., Yadav, S. K., Maurya, S. K., & Kumar, S. (2020). Caprolactam: A versatile lactam with emerging biological applications. Bioorganic Chemistry, 96, 103610.

Li, Q., Wang, J., & Zhang, H. (2019). Comparative Analysis of Bioactive Compounds in Plant Species: Implications for Bioactivity. Journal of Agricultural and Food Chemistry, 67(10), 2857-2867.

Olsson, M. E., Andersson, R., & Nyström, L. (2019). Mass spectrometry-based analysis of plant secondary metabolites: Application to bioactive compounds in natural products. Journal of Mass Spectrometry, 54(5), 307-322.

Peng, Y., Zhang, Y., Xu, Q., & Li, J. (2010). Effects of sinapic acid on oxidative stress and antioxidant capacity in broilers. Poultry Science, 89(3), 479–485. https://doi.org/10.3382/ps.2009-00241

Rita W., Definiati N., Zurina R., Susilo E., & Parwito. 2024a. Study of Morphology, Nutrition and Bioactive Compounds at Two Accessions Marigold (Tagetes erecta) in Kepahiang Regency. Interantional Journal of Integrative Sciences (IJIS). 3 (11) 1181-1190.

Rita W., Chozin M., Sumardi., & Dwatmadji. 2024b. Identification of Phenotypic, Nutrition and Bioactive Compounds of Marigold (Tagetes erecta) in Bengkulu Province, Indonesia as Poultry Feed Material. Interantional Journal of Integrative Sciences (IJIS). 3 (12) 1427-442.

Rohman, A., Suryani, A., & Hidayati, N. (2019). Bioactive Compounds in Plants: GC-MS Identification and Antioxidant Activity. Journal of Ethnopharmacology, 245, 112-123.

Sánchez, A. H., Fernández, M. D., & García, A. L. (2014). GC-MS analysis of bioactive compounds in plant extracts: The importance of area percentage calculation. Journal of Analytical Chemistry, 69(6), 1-10.

Sharangi, A. B. Acharya, S. K., 2009. Stewardship and Conservation in Agriculture: The Small farm Reality.

Smith, T. J. (2000). Squalene: Potential chemopreventive agent. Expert Opinion on Investigational Drugs, 9(8), 1841–1848. https://doi.org/10.1517/13543784.9.8.1841

Surai, P. F. (2002). Selenium in poultry nutrition 1. Antioxidant properties, deficiency and toxicity. World's Poultry Science Journal, 58(3), 333–347. https://doi.org/10.1079/WPS20020024

Wina, E., Hidayat, C., & Sopiyana, S. 2016Manfaat Senyawa Bioaktif Dedak Padi untuk Pakan Fungsional Ternak Ayam.

Windisch, W., Schedle, K., Plitzner, C., & Kroismayr, A. (2008). Use of phytogenic products as feed additives for swine and poultry. Journal of Animal Science, 86(14_suppl), E140–E148. https://doi.org/10.2527/jas.2007-0459

Zhao, Y., Wang, X., & Liu, Z. (2018). GC-MS Analysis and Visualization of Bioactive Compounds in Plant Extracts. Analytical Chemistry, 90(3), 589-596.

Downloads

Published

2025-05-22

How to Cite

Wismalinda Rita, Mohammad Chozin, Sumardi, & Dwatmadji. (2025). Phytochemical Content Analysis of 4 Marigold (Tagetes Erecta) Accsessions in Bengkulu Province Which has the Potential as a Poultry Feed Supplement. International Journal of Integrative Sciences, 4(5), 965–980. https://doi.org/10.55927/ijis.v4i5.254

Issue

Section

Articles